Humboldt-Universität zu Berlin, 10115 Berlin, Germany
Ph.D in veterinary science, M.sc in applied microbiology, veterinary scientist, microbiologist, microbial biofilm researcher. I was a Junior postdoctoral at University of California Santa Cruz, focusing on survival strategies of Helicobacter pylori, with a particular focus on biofilm and genes associated with this mode of growth. I'm currently a a senior postdoctoral at Humboldt-Universität zu Berlin developing new tools to study morphogenetic movements in macrocolony biofilm.
Humboldt-Universität zu Berlin
Since August 2019
Investigating E. coli macrocolony biofilms and their architectures. Role of cellulose and Curli. Interdisciplinary project (Cluster Matters of Activity)
Joining the laboratory of Prof. Regine Hengge
Teaching Molecular Mikrobiologie Modul BXY-36. Wintersemerster (2019-2020)
Guest lecture: Biofilm microbial shelters. Feb. 2020. Humboltd-Universität zu Berlin
Univeristy of California, Santa Cruz
to June 2019
Investigating Helicobacter pylori biofilm formation and its potential role in pathogenesis in the lab of Dr. Karen Ottemann. I'm using transcriptomic and genetic approaches to identify genes associated with H. pylori biofilm growth. We also use mouse-models for H. pylori infections and imaging approaches (CSLM and SEM) to characterize the biofilm structure its components.
1. Effect of omeprazole on gland colonization of Helicobacter pylori in a Murine model. A. Clark, S. Hathroubi and K. M. Ottemann. (In preparation)
2. Genetic requirements and transcriptomics of Helicobacter pylori biofilm formation on abiotic and biotic surfaces. S. Hathroubi, S. Hu and K. M Ottemann. 2020. NPJ Biofilms and Microbiomes. (accepted for publication)
3. Helicobacter pylori biofilm confers antibiotic tolerance in part via a protein-dependent mechanism. S. Hathroubi, J. Zerebinski, A. Clarke and K. M Ottemann. 2020. Antibiotics (Basel). (Jun 24;(6):355)
4. Helicobacter pylori biofilm involves a multigene stress-biased response, including a structural role for flagella. S. Hathroubi, J. Zerebinski and K. M Ottemann. mBio. 2018. (Oct 30;9(5):e011973-18)
5. Helicobacter pylori biofilm formation and its potential role in pathogenesis. S. Hathroubi, S. L. Servetas, I. Windham, D. S. Merrell, K. M Ottemann. 2018. Microbiology and Molecular Biology Reviews. (Published, doi: 10.1128/MMBR.00001-18)
Since May 2018
Teaching Molekulare Mikrobiologie (Modul BXY-36,5. Semester)
Institut für Biologie, AG Mikrobiologie, Humboldt-Universität zu Berlin, Berlin, Germany- 2019-2020 and 2020-2021
Seminar training, reading and lectures.
Lectures: Bacterial biofilm and Bacterial pathogenicity
Guest lecture microbiology and pathogenesis for undergraduates and graduates: Biofilm, the social life of microorganisms. University of California, Santa Cruz (Winter-Spring session 2018 and 2019)
Microbiology and pathogenesis METX 119-02 online, summer session online classes: Biofilm, the social life of microorganisms. Univeristy of California, Santa Cruz (Summer 2018)
Teaching course microbial pathogenesis 2 for Master’s degree, veterinary science: Surface polysaccharides and envelope (Part_1. Microbial envelope and Part_2. Surface polysaccharides). Université de Montréal (Fall session 2015)
Laboratory demonstrator for Master’s degree, veterinary science students: Basic practical microbiology. Université de Montréal. (2012- 2015)
University of California, Santa Cruz
Supervising undergrad student Yasmine E. (University of California, Santa Cruz) - August 2018 - July 2019
Supervising Ph.D student Aaron C. (University of California, Santa Cruz) - October 2017 - July 2019
Supervising undergrad student Julia Z. (University of California, Santa Cruz) - October 2017-September 2018
Supervising summer student Tyler Dewitt (University of California, Santa Cruz) - June-July 2017
Supervising student internship Sarah-Eve Fontaine-Gosselin (University of Montreal) Bachelor of Life Science, Sherbrooke University) - January-May 2014
# Invited Speaker workshop ‘Material as Environmental Device’, Berlin; Germany. 10-11 September 2020
# Invited speaker to 5th Workshop on bacterial and fungal biofilms, Ghent University conference Center. May 2019. Unraveling the mystery of Helicobacter pylori biofilm.
# Invited speaker to INRS-INSTITUT ARMAND-FRAPPIER October 2018. Helicobacter pylori biofilm and the unexpected role of flagella.
# Invited speaker to 2nd Technology Seminar-SandBox (UCSC_Startup incubator) September 2018. Targeting the Helicobacter pylori impenetrable biofilm.
# PBSE Research Conference 2018, Felton, California, USA. Unexpected role played by flagella during biofilm formation.
# UC Biosafety officer meeting 2018, Santa Cruz, USA. New Insights into Helicobacter pylori biofilm.
# WCBP Annual conference, 2017, Asilomar, California, USA. Novel genes associated with Helicobacter pylori biofilm growth.
# CRIPA symposium, 2016, St-Hyacinthe, Québec, Canada. Actinobacillus pleuropneumoniae biofilms mitigate innate immune response.
# ASM Biofilm, 2015, Chicago, USA. Actinobacillus pleuropneumoniae biofilms induce lower response of porcine alveolar macrophages.
# CRIPA symposium, 2015, St-Hyacinthe, Québec, Canada. Effect of sub-inhibitory concentrations of penicillin G on Actinobacillus pleuropneumoniae biofilm formation.
# Phd in 180sec (3MTMC) 2014, Science on stage, Faculté de médecine vétérinaire, St-Hyacinthe, Québec Canada. Best presentation 3rd prize. Rôle du biofilm dans la pathogénicité d’Actinobacillus pleuropneumoniae.
# Congrès BiSP 2013, Université Laval, Québec, Canada. Rôle des polysaccharides de surface dans la formation du biofilm chez Actinobacillus pleuropneumoniae.
Reviser for international Peer-reviewed Journal
# Referee/reviewer for Frontiers Microbiology
# Referee reviewer for Journal of Infection in Developing Countries
# Referee reviewer for Clinical and Experimental Gastroenterology
# Referee reviewer for Infection and Drug Resistance
Microbiology Research Internship
Groupe de recherche sur les maladies infectieuses du porc (GREMIP)
Assist students and teachers in location of print and non-print resouces
Check books and materials in and out to students and teachers using the computerized circulation system
Monitor student use of the library
Assist in maintaining the appearance of the library
Université de Montréal
Since September 2012
Canada - Québec
Monitoring students during the writing of tests/exams
PhD in Veterinary Science, Microbiology and Infectious Disease
Faculté de médecine vétérinaire, Université de Montréal
to September 2016
Study of micro-organisms in their natural environment. Development of techniques for culturing bacteria form biofilms. Effect of antibiotics on biofilm. Immune response towards biofilms. Phenotypic changes in biofilms cells.
1_ Actinobacillus pleuropneumoniae: Role of biofilm in pathogenicity and potential impact for vaccination development. S. Hathroubi, A. Loera, J.T. Bossé, P.R. Langford and M. Jacques. 2017. Animal Health Research Review. (Published,7:1-14)
4_ Surface polysaccharide mutants reveal that absence of O antigen reduces biofilm formation of Actinobacillus pleuropneumoniae. S. Hathroubi, M.A. Hancock, J.T. Bossé, P.R. Langford, Y.D.N Tremblay, J. Labrie and M. Jacques. 2015. Infection and Immunity
5_ Sub-inhibitory concentrations of penicillin G induce biofilm formation by field isolates of Actinobacillus pleuropneumoniae. S. Hathroubi, S-È. Fontaine-Gosselin, Y.D.N. Tremblay, J. Labrie and M. Jacques. 2015. Vet Microbiology (http://www.ncbi.nlm.nih.gov/pubmed/26130517).
6_ Les biofilms bactériens : leur importance en santé animale et en santé publique. Y.D.N. Tremblay, S. Hathroubi and M. Jacques. 2014. Canadian Journal of Veterinary Research (http://www.ncbi.nlm.nih.gov/pubmed/24688172).
Research Workshop Viral biofilms
Study of Actinobacillus pleuropneumoniae pathogen pork biofilm
Mutagenesis and characterization of biofilm mutant
Communication during PhD program
The "viral biofilms" Out Of Context Seminar
Research Workshop pharmacokinetic / pharmacodynamic
Certificate '' Bacteria biofilms in chronic infections''
Faculty of Health Sciences, University of Copenhagen
to November 2012
Distinguish biofilms from planktonic bacteria both in nature and in medical related situations. Examine health care problems caused by infections involving bacterial biofilms. Design and develop biofilm models. Strategies for future research.
Training/Certification program for animal experimentation
Université de Montréal
Since September 2011
The Ethics of animal experimentation gives me the right to experiment on animals ifor laboratory research
Master Applied Microbiology, Environment and Health
Université Pierre Et Marie Curie, Paris 6, France
to June 2010
A good understanding of microbiology ; bacteriology, virology, parasitology, environmental quality and health.
Project communication: GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF BIOFILM PRODUCTION IN PSEUDOMONAS AEROGINOSA STRAINS IN THE BONE MARROW TRANSPLANT CENTER OF TUNIS, TUNISIA
Training the Oceanological Observatory Banyuls-sur-mer
Biodiversity and biotechnology to microorganisms
Bachelor of Life Sciences
Université Pierre et Marie Curie, Paris 6 France
to June 2008
Scientific knowledge of biology and the general organisation of the animal and vegetable kingdoms as well as the functioning of organisms, from the molecular level to integrated biological systems.
Microscopic identification, biochemical testing of bacteria
Antibiotic susceptibility tests and serotyping assay
Biofilms culture in static and dynamic conditions
Enzymatic and chemicals treatments on biofilms
Swarming et twitching motility tests
Cell culture, macrophages culture
Cytokines detections (PCR and ELISA)
PBMC isolation and proliferation
Gastric gland isolation and imaging
Biofilm staining and EPS detection
3D analysis (biomass, volume and thickness)
SEM microscopy of biofilm and flagella detection
DNA and RNA extraction, PCR and RT-PCR
EPS extraction (eDNA...)
Immunodetection of extracellular polymeric substance (EPS)