Your browser is outdated!

To ensure you have the best experience and security possible, update your browser. Update now

×

Skander Hathroubi

Ph.D in veterinary science, microbiologist and biofilm researcher

Skander Hathroubi
Contact
+4917669168559 skander.hathroubi@hu-berlin.de
Humboldt-Universität zu Berlin, 10115 Berlin, Germany
About Me
Ph.D in veterinary science, M.sc in applied microbiology, scientist, microbiologist, microbial biofilm researcher. I was a Junior postdoctoral at University of California Santa Cruz, focusing on survival strategies of Helicobacter pylori, with a particular focus on biofilm and genes associated with this mode of growth. I'm currently a postdoctoral research assistant at Humboldt-Universität zu Berlin.
Experience
  • E. coli chromosomal toxin antitoxin systems identification by PCR
  • Sequencing
  • Phylogenetic analyses
Education

PhD in Veterinary Science, Microbiology and Infectious Disease

Faculté de médecine vétérinaire, Université de Montréal

May 2011 to September 2016
Study of micro-organisms in their natural environment. Development of techniques for culturing bacteria form biofilms. Effect of antibiotics on biofilm. Immune response towards biofilms. Phenotypic changes in biofilms cells.
GPA 3.85/4.3

Publications:

1_ Actinobacillus pleuropneumoniae: Role of biofilm in pathogenicity and potential impact for vaccination development. S. Hathroubi, A. Loera, J.T. Bossé, P.R. Langford and M. Jacques. 2017. Animal Health Research Review. (Published,7:1-14)

2_ Actinobacillus pleuropneumoniae biofilms induce lower response of porcine alveolar macrophages. S. Hathroubi, C. Provost, C. A. Gagnon and M. Jacques. Innate immunity
(http://www.ncbi.nlm.nih.gov/pubmed/27226465)

3_ Biofilms: Bacterial shelters against antibiotics. S. Hathroubi, M.A Mekni, P. Domenico, D. Nguyen and M. Jacques. Microbial Drug Resistance
(http://www.ncbi.nlm.nih.gov/pubmed/27214143)

4_ Surface polysaccharide mutants reveal that absence of O antigen reduces biofilm formation of Actinobacillus pleuropneumoniae. S. Hathroubi, M.A. Hancock, J.T. Bossé, P.R. Langford, Y.D.N Tremblay, J. Labrie and M. Jacques. 2015. Infection and Immunity
(http://www.ncbi.nlm.nih.gov/pubmed/26483403).

5_ Sub-inhibitory concentrations of penicillin G induce biofilm formation by field isolates of Actinobacillus pleuropneumoniae. S. Hathroubi, S-È. Fontaine-Gosselin, Y.D.N. Tremblay, J. Labrie and M. Jacques. 2015. Vet Microbiology (http://www.ncbi.nlm.nih.gov/pubmed/26130517).

6_ Les biofilms bactériens : leur importance en santé animale et en santé publique. Y.D.N. Tremblay, S. Hathroubi and M. Jacques. 2014. Canadian Journal of Veterinary Research (http://www.ncbi.nlm.nih.gov/pubmed/24688172).
Research Workshop Viral biofilms
Study of Actinobacillus pleuropneumoniae pathogen pork biofilm
Mutagenesis and characterization of biofilm mutant

Communication during PhD program
The "viral biofilms" Out Of Context Seminar
Research Workshop pharmacokinetic / pharmacodynamic

Certificate '' Bacteria biofilms in chronic infections''

Faculty of Health Sciences, University of Copenhagen

September 2012 to November 2012
Distinguish biofilms from planktonic bacteria both in nature and in medical related situations. Examine health care problems caused by infections involving bacterial biofilms. Design and develop biofilm models. Strategies for future research.

Training/Certification program for animal experimentation

Université de Montréal

Since September 2011
The Ethics of animal experimentation gives me the right to experiment on animals ifor laboratory research

Master Applied Microbiology, Environment and Health

Université Pierre Et Marie Curie, Paris 6, France

September 2008 to June 2010
A good understanding of microbiology ; bacteriology, virology, parasitology, environmental quality and health.
Project communication: GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF BIOFILM PRODUCTION IN PSEUDOMONAS AEROGINOSA STRAINS IN THE BONE MARROW TRANSPLANT CENTER OF TUNIS, TUNISIA
Training the Oceanological Observatory Banyuls-sur-mer
Biodiversity and biotechnology to microorganisms

Bachelor of Life Sciences

Université Pierre et Marie Curie, Paris 6 France

September 2005 to June 2008
Scientific knowledge of biology and the general organisation of the animal and vegetable kingdoms as well as the functioning of organisms, from the molecular level to integrated biological systems.
Skills

Microbiology

  • Microscopic identification, biochemical testing of bacteria
  • Antibiotic susceptibility tests and serotyping assay
  • Biofilms culture in static and dynamic conditions
  • Enzymatic and chemicals treatments on biofilms
  • Swarming et twitching motility tests
  • Drip flow reactor systems
  • MBEC assay

Cell biology

  • Cell culture
  • Macrophages culture
  • Cytokines detections
  • PBMC isolation and proliferation
  • Gastric gland isolation

Confocal microscopy

  • Biofilm staining and EPS detection
  • 3D analysis (biomass, volume and thickness)
  • EPS detection

Molecular biology

  • DNA and RNA extraction
  • RNA-seq
  • Site-directed mutagenesis
  • EPS extraction (eDNA...)
  • PCR and RT-PCR
  • Lipid A micro-extractions
  • LPS extractions
  • Immunodetection of extracellular polymeric substance (EPS)

Animal experiment

  • Mice infection and treatments
  • Gastric gland isolation
Interests

Informatics

  • Photography; photo treatment Photoshop, ACDSee
  • Adobe InDesign

Foreign trips

  • 18 countries visited
  • Road trips cross-country (Canada, USA)

Cryptocurrency

  • Ethereum (ETH) mining
  • Trading Lumen Stellar, Ripple and Litecoin
Download Download