Skander Hathroubi
Ph.D in veterinary science, microbiologist and biofilm researcher
Contact
+4917669168559
skander.hathroubi@hu-berlin.de
Humboldt-Universität zu Berlin, 10115 Berlin, Germany
About Me
Ph.D in veterinary science, M.sc in applied microbiology, veterinary scientist, microbiologist, microbial biofilm researcher. I was a Junior postdoctoral at University of California Santa Cruz, focusing on survival strategies of Helicobacter pylori, with a particular focus on biofilm and genes associated with this mode of growth. I'm currently a a senior postdoctoral at Humboldt-Universität zu Berlin developing new tools to study morphogenetic movements in macrocolony biofilm.
Experience
Mentorship experiences
University of California, Santa Cruz
Since 2017
- Supervising undergrad student Yasmine E. (University of California, Santa Cruz) - August 2018 - July 2019
- Supervising Ph.D student Aaron C. (University of California, Santa Cruz) - October 2017 - July 2019
- Supervising undergrad student Julia Z. (University of California, Santa Cruz) - October 2017-September 2018
- Supervising summer student Tyler Dewitt (University of California, Santa Cruz) - June-July 2017
- Supervising student internship Sarah-Eve Fontaine-Gosselin (University of Montreal) Bachelor of Life Science, Sherbrooke University) - January-May 2014
Education
PhD in Veterinary Science, Microbiology and Infectious Disease
Faculté de médecine vétérinaire, Université de Montréal
May 2011
to September 2016
Study of micro-organisms in their natural environment. Development of techniques for culturing bacteria form biofilms. Effect of antibiotics on biofilm. Immune response towards biofilms. Phenotypic changes in biofilms cells.
GPA 3.85/4.3
Publications:
1_ Actinobacillus pleuropneumoniae: Role of biofilm in pathogenicity and potential impact for vaccination development. S. Hathroubi, A. Loera, J.T. Bossé, P.R. Langford and M. Jacques. 2017. Animal Health Research Review. (Published,7:1-14)
2_ Actinobacillus pleuropneumoniae biofilms induce lower response of porcine alveolar macrophages. S. Hathroubi, C. Provost, C. A. Gagnon and M. Jacques. Innate immunity
(http://www.ncbi.nlm.nih.gov/pubmed/27226465)
3_ Biofilms: Bacterial shelters against antibiotics. S. Hathroubi, M.A Mekni, P. Domenico, D. Nguyen and M. Jacques. Microbial Drug Resistance
(http://www.ncbi.nlm.nih.gov/pubmed/27214143)
4_ Surface polysaccharide mutants reveal that absence of O antigen reduces biofilm formation of Actinobacillus pleuropneumoniae. S. Hathroubi, M.A. Hancock, J.T. Bossé, P.R. Langford, Y.D.N Tremblay, J. Labrie and M. Jacques. 2015. Infection and Immunity
(http://www.ncbi.nlm.nih.gov/pubmed/26483403).
5_ Sub-inhibitory concentrations of penicillin G induce biofilm formation by field isolates of Actinobacillus pleuropneumoniae. S. Hathroubi, S-È. Fontaine-Gosselin, Y.D.N. Tremblay, J. Labrie and M. Jacques. 2015. Vet Microbiology (http://www.ncbi.nlm.nih.gov/pubmed/26130517).
6_ Les biofilms bactériens : leur importance en santé animale et en santé publique. Y.D.N. Tremblay, S. Hathroubi and M. Jacques. 2014. Canadian Journal of Veterinary Research (http://www.ncbi.nlm.nih.gov/pubmed/24688172).
Research Workshop Viral biofilms
Study of Actinobacillus pleuropneumoniae pathogen pork biofilm
Mutagenesis and characterization of biofilm mutant
Communication during PhD program
The "viral biofilms" Out Of Context Seminar
Research Workshop pharmacokinetic / pharmacodynamic
GPA 3.85/4.3
Publications:
1_ Actinobacillus pleuropneumoniae: Role of biofilm in pathogenicity and potential impact for vaccination development. S. Hathroubi, A. Loera, J.T. Bossé, P.R. Langford and M. Jacques. 2017. Animal Health Research Review. (Published,7:1-14)
2_ Actinobacillus pleuropneumoniae biofilms induce lower response of porcine alveolar macrophages. S. Hathroubi, C. Provost, C. A. Gagnon and M. Jacques. Innate immunity
(http://www.ncbi.nlm.nih.gov/pubmed/27226465)
3_ Biofilms: Bacterial shelters against antibiotics. S. Hathroubi, M.A Mekni, P. Domenico, D. Nguyen and M. Jacques. Microbial Drug Resistance
(http://www.ncbi.nlm.nih.gov/pubmed/27214143)
4_ Surface polysaccharide mutants reveal that absence of O antigen reduces biofilm formation of Actinobacillus pleuropneumoniae. S. Hathroubi, M.A. Hancock, J.T. Bossé, P.R. Langford, Y.D.N Tremblay, J. Labrie and M. Jacques. 2015. Infection and Immunity
(http://www.ncbi.nlm.nih.gov/pubmed/26483403).
5_ Sub-inhibitory concentrations of penicillin G induce biofilm formation by field isolates of Actinobacillus pleuropneumoniae. S. Hathroubi, S-È. Fontaine-Gosselin, Y.D.N. Tremblay, J. Labrie and M. Jacques. 2015. Vet Microbiology (http://www.ncbi.nlm.nih.gov/pubmed/26130517).
6_ Les biofilms bactériens : leur importance en santé animale et en santé publique. Y.D.N. Tremblay, S. Hathroubi and M. Jacques. 2014. Canadian Journal of Veterinary Research (http://www.ncbi.nlm.nih.gov/pubmed/24688172).
Research Workshop Viral biofilms
Study of Actinobacillus pleuropneumoniae pathogen pork biofilm
Mutagenesis and characterization of biofilm mutant
Communication during PhD program
The "viral biofilms" Out Of Context Seminar
Research Workshop pharmacokinetic / pharmacodynamic
Certificate '' Bacteria biofilms in chronic infections''
Faculty of Health Sciences, University of Copenhagen
September 2012
to November 2012
Distinguish biofilms from planktonic bacteria both in nature and in medical related situations. Examine health care problems caused by infections involving bacterial biofilms. Design and develop biofilm models. Strategies for future research.
Training/Certification program for animal experimentation
Université de Montréal
Since September 2011
The Ethics of animal experimentation gives me the right to experiment on animals ifor laboratory research
Master Applied Microbiology, Environment and Health
Université Pierre Et Marie Curie, Paris 6, France
September 2008
to June 2010
A good understanding of microbiology ; bacteriology, virology, parasitology, environmental quality and health.
Project communication: GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF BIOFILM PRODUCTION IN PSEUDOMONAS AEROGINOSA STRAINS IN THE BONE MARROW TRANSPLANT CENTER OF TUNIS, TUNISIA
Training the Oceanological Observatory Banyuls-sur-mer
Biodiversity and biotechnology to microorganisms
Project communication: GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF BIOFILM PRODUCTION IN PSEUDOMONAS AEROGINOSA STRAINS IN THE BONE MARROW TRANSPLANT CENTER OF TUNIS, TUNISIA
Training the Oceanological Observatory Banyuls-sur-mer
Biodiversity and biotechnology to microorganisms
Bachelor of Life Sciences
Université Pierre et Marie Curie, Paris 6 France
September 2005
to June 2008
Scientific knowledge of biology and the general organisation of the animal and vegetable kingdoms as well as the functioning of organisms, from the molecular level to integrated biological systems.
Skills
Microbiology
- Microscopic identification, biochemical testing of bacteria
- Antibiotic susceptibility tests and serotyping assay
- Biofilms culture in static and dynamic conditions
- Enzymatic and chemicals treatments on biofilms
- Swarming et twitching motility tests
- Drip flow reactor systems
- MBEC assay
Cell biology
- Cell culture
- Macrophages culture
- Cytokines detections
- PBMC isolation and proliferation
- Gastric gland isolation
Confocal microscopy
- Biofilm staining and EPS detection
- 3D analysis (biomass, volume and thickness)
- EPS detection
Molecular biology
- DNA and RNA extraction
- RNA-seq
- Site-directed mutagenesis
- EPS extraction (eDNA...)
- PCR and RT-PCR
- Lipid A micro-extractions
- LPS extractions
- Immunodetection of extracellular polymeric substance (EPS)
Animal experiment
- Mice infection and treatments
- Gastric gland isolation
Interests
Informatics
- Photography; photo treatment Photoshop, ACDSee
- Adobe InDesign
Foreign trips
- 18 countries visited
- Road trips cross-country (Canada, USA)
Cryptocurrency
- Ethereum (ETH) mining
- Trading Lumen Stellar, Ripple and Litecoin
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